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You are here:Home » Antibodies » Abs to Receptors, Estrogen » Anti -Estrogen Receptor, alpha, phosphorylated (Ser167) (ER-alpha, ERa, ER, ESR1, ESR, Estradiol Receptor, Nuclear Receptor Subfamily 3 Group A Member 1, NR3A1)

Anti -Estrogen Receptor, alpha, phosphorylated (Ser167) (ER-alpha, ERa, ER, ESR1, ESR,
Estradiol Receptor, Nuclear Receptor Subfamily 3 Group A Member 1, NR3A1)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Purified B
Estrogen receptor a (ERa), a member of the steroid receptor superfamily, contains highly conserved DNA binding (DBD) and ligand binding domains (LBD) (1). Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, (espectively), ERa regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery (2). Phosphorylation provides an important mechanism to regulate ERa activity (3,4). ERa is phosphorylated on multiple sites (5). Ser104, 106, 118 and 167 are located in the aminoterminal transcription activation function domain AF-1, and
phosphorylation of these serines plays an important role in regulating ERa activity. Ser118 may be the substrate of the transcription regulatory kinase CDK7 (5). Ser167 may be phosphorylated by p90RSK and Akt (4,6). Phosphorylation of Ser167 may confer tamoxifen resistance in breast cancer patients (4).
Catalog #E3565-10A1
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG
Clone No11C1030
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, less than 0.02% sodium azide, 50% glycerol.
PurityPurified
ImmunogenSynthetic phosphopeptide corresponding to residues surrounding Ser167 of human estrogen receptor a.
SpecificityRecognizes endogeneous levels of ERa, phosphorylated at (Ser167). Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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