Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Receptors, Estrogen » Anti -Estrogen Receptor, alpha, phosphorylated (Ser118) (ER)

Anti -Estrogen Receptor, alpha, phosphorylated (Ser118) (ER)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B IH
Estrogen receptor alpha (ER alpha), a member of the steroid receptor superfamily, contains highly conserved DNA binding (DBD) and ligand binding domains (LBD) (1). Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ER alpha regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery (2). Phosphorylation provides an important mechanism to regulate ER alpha activity (3,4). ER alpha is phosphorylated on multiple sites (5). Serines 104, 106, 118 and 167 are located in the N-terminal transcription activation function domain AF-1, and phosphorylation of these serines plays an important role in regulating ER alpha activity. Ser118 may be the substrate of the transcription regulatory kinase cdk7 (5). Ser167 may be phosphorylated by p90RSK and Akt (4,6). Phosphorylation of Ser167 may confer tamoxifen resistance in breast cancer patients (4).
Catalog #E3565-10C
Recommended Dilution Western Blotting: 1:1000, incubate membrane with diluted antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Immunohistochemistry (Paraffin): 1:50
Optimal dilutions to be determined by the researcher.
Storage and Stability May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No3A15
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
PurityPurified.
ImmunogenSynthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser118 of human ER alpha.
SpecificityDetects endogenous levels of estrogen receptor alpha only when phosphorylated at Ser118. It does not crossreact with phosphorylated estrogen receptor beta. Species Crossreactivity: Mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links