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You are here:Home » Antibodies » Abs to Receptors, Estrogen » Anti -Estrogen Receptor, alpha, phosphorylated (Ser118) (ER)

Anti -Estrogen Receptor, alpha, phosphorylated (Ser118) (ER)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Affinity Purified B
Catalog #E3565-10G
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:2000 detects phospho-estrogen receptor (Ser118) in RIPA lysates from MCF-7 cells treated with estradiol and EGF, and recombinant estrogen receptor phosphorylated in vitro with MAP Kinase 2/Erk2, active. Lysates from estradiol and EGF treated MCF-7 cells (lanes 1 and 2) or recombinant estrogen receptor protein phosphorylated with MAPK2/Erk2 (lanes 3 and 4) were resolved by electrophoresis and transferred to nitrocellulose. Following blocking of the membrane, the nitrocellulose was either treated with lambda phosphatase (lanes 1 and 3) or left untreated (lanes 2 and 4), then probed with anti-phospho- Estrogen Receptor (Ser118) (1:2000 dilution). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No4H323
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 0.2M Tris-glycine, pH 7.4, 0.15M NaCl, 100uM EDTA, 0.05% sodium azide, before the addition of glycerol to 30%.
PurityPurified by Protein A affinity chromatography.
ImmunogenKLH-conjugated, synthetic peptide containing the sequence …QLpSPF… in which pS corresponds to phosphoserine at residue 118 of
human estrogen receptor alpha. The immunizing sequence has 12/13 identical amino acids in camel, mouse, porcine, and Syrian hamster.
SpecificityRecognizes phospho-estrogen receptor alpha (Ser118), Mr 66kD. Species Crossreactivity: Human. Others not tested. Predicted to crossreact with camel, mouse, porcine and Syrian hamster based on sequence homology.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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