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You are here:Home » Antibodies » Abs to Receptors, Estrogen » Anti -Estrogen Receptor, alpha, phosphorylated (Ser305) (ERa)

Anti -Estrogen Receptor, alpha, phosphorylated (Ser305) (ERa)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Estrogen Receptor alpha is a 65kD protein and a member of the steroid family of nuclear receptors. Estrogen Receptor alpha is a ligand-activated transcription factor that, when bound to estrogen, induces a conformational change that allows dimerization and binding to estrogen response element sequences. When bound to DNA, Estrogen Receptor alpha can positively or negatively regulate gene transcription.
Catalog #E3565-10W
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:100-1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%) Freeze at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic phosphopeptide, which represented a portion of human Estrogen Receptor Alpha (GeneID 2099) around serine 305 according to the numbering given in entry NP_000116.2.
SpecificityRecognizes human ERa when phosphorylated at Ser305.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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