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You are here:Home » Antibodies » Antibodies-Receptors, Estrogen » Anti -Estrogen-Related Receptor, alpha (ERRa, NR3B1, NR3 Steroid Receptor)

Anti -Estrogen-Related Receptor, alpha (ERRa, NR3B1, NR3 Steroid Receptor)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IH
Estrogen-related receptor alpha is a nuclear receptor protein related to the Estrogen receptor. It is reported to interact with estrogen and similar steroids and functions as a transcriptional activator. In mouse, ERR alpha is expressed in many adult and embryonic tissues (particularly at the onset of ossification) as well as in several osteoblast cell lines. ERR alpha expression has been documented in mouse in brain, spinal cord, pituitary gland, heart, intestine, bone, brown adipose tissue, heart, uterus, cervix, nerve, skeletal muscle, and vagina. ESTs have been isolated from human tissue libraries, including cancerous blood, brain, breast, cervix, colon, duodenum, eye, head/neck, kidney, liver, lung, ovary, pancreas, skeletal muscle, skin, stomach, and uterus, and normal adrenal, blood, brain, colon, embryo, eye, head/neck, heart, kidney, prostate, skeletal muscle, testis, and uterus.
Catalog #E3565-13B
ApplicationsSuitable for use in Immunohistochemistry, Western Blot, Immunoprecipitation, Electrophoretic Mobility Shift Assay. Other applications not tested.
Recommended DilutionWestern Blot: 0.5-2.0ug/ml evaluated in RIPA lysates from HeLa nuclear extracts.
Immunohistochemistry: Reported to detect ERR alpha in mouse embryo, mouse liver, and hampster liver and heart sections.
Optimal dilutions to be determined by the researcher.
Molecular Weight 53kD
Positive Control IncludedH1838: HeLa Nuclear Extract. Load 20ug of reduced lysate per lane for Western Blot.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
FormSupplied as a liquid in 0.1M Tris-glycine, pH7.4, 0.15M NaCl, 0.05% sodium azide, 30% glycerol.
PurityPurified by Protein A affinity chromatography.
ImmunogenOvalbumin-conjugated, synthetic peptide corresponding to amino acids 339-364, SVHIEDAEAVEQLREALHEALLEYEA, of human Estrogen Receptor-Related alpha.
SpecificityRecognizes human ERR alpha (Estrogen-related Receptor alpha). Species Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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