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You are here:Home » Antibodies » Abs to Receptors, Estrogen » Anti -Estrogen-Related Receptor, alpha (ERRa, NR3B1, NR3 Steroid Receptor)

Anti -Estrogen-Related Receptor, alpha (ERRa, NR3B1, NR3 Steroid Receptor)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP FC
This protein is a nuclear receptor that is closely related to the estrogen receptor. This protein acts as a site specific transcription regulator and has been also shown to interact with estrogen and the transcripton factor TFIIB by direct protein-protein contact. The binding and regulatory activities of this protein have been demonstrated in the regulation of a variety of genes including lactoferrin, osteopontin, medium chain acyl coenzyme A dehydrogenase (MCAD) and thyroid hormone receptor genes. In mouse, ERR alpha is expressed in many adult and embryonic tissues (particularly at the onset of ossification) as well as in several osteoblast cell lines. ERR alpha expression has been documented in mouse in brain, spinal cord, pituitary gland and heart.
Catalog #E3565-13D
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Flow Cytometry. Other applications not tested.
Recommended DilutionWestern Blot: 1:5000
Flow Cytometry: 1:20
Immunoprecipitation: 1:40
Optimal dilutions to be determined by the researcher.
Positive ControlHeLa cell lysate.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG
Clone No10F639
HostRabbit
SourceMouse
Concentration1.0mg/ml
FormSupplied as a liquid in PBS 50 mM Tris-Glycine, pH 7.4, 0.15M NaCl, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
PuritySupernatant
ImmunogenSynthetic peptide corresponding to residues near the N-terminus of human Estrogen Related Receptor alpha.
SpecificityRecognizes human Estrogen Related Receptor alpha. Species crossreactivity: mouse, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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