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You are here:Home » Antibodies » Abs to Receptors, Estrogen » Anti -Estrogen Receptor, beta (ERb)

Anti -Estrogen Receptor, beta (ERb)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Catalog #E3565-16E
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionImmunoblot Analysis: 2-4ug/ml of this lot detected Estrogen Receptor b (ERb) in RIPA lysates from MCF-7 cell lysate.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide (YAEPQKSPWCEARSLEHT) representing aa54-71 of rat and mouse estrogen receptor beta and aa46-63 of human estrogen receptor beta.
SpecificityRecognizes the 53kD estrogen receptor b splice variant in cell lysates. Non-specific doublet detected at 75-77kD and an additional band at 47kD, likely an ERb degradation product. Also recognizes recombinant ERb, long form, at 59kD. Does not detect estrogen receptor a. Species Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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