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You are here:Home » Antibodies » Abs to Receptors, Estrogen » Anti -Estrogen Receptor, beta (ERb)

Anti -Estrogen Receptor, beta (ERb)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
Catalog #E3565-16W
ApplicationsSuitable for use in Western Blot, Immunohistochemistry (paraffin), and Gel shift assay. Other applications not tested.
Recommended DilutionGel Shift: 1-5ug/ml
Immunohistochemistry: 1-2ug/ml.. IH staining of ER beta in rat cerebellum results in cytoplasmic staining in Purkinje neurons.
Western Blot: 1-2ug/ml. In rat brain homogenate, detects an ~55kD protein representing ER beta
Optimal dilutions to be determined by the researcher.
Cellular Localization Nuclear
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in PBS, containing 1mg/ml BSA and 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide: AEPQKSPWCEARSLEH, corresponding to amino acids 55-70 of rat Estrogen Receptor beta.
SpecificityRecognizes human estrogen receptor beta. Species Crossreactivity: rat, mouse and primate.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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