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You are here:Home » Antibodies » Abs to Receptors, Estrogen » Anti -Estrogen Receptor, beta (ERb)

Anti -Estrogen Receptor, beta (ERb)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Estrogen receptor (ER) is a member of the steroid-receptor family. Unlike protein growth factors that bind to receptors on the cell surface and activate signal-transduction cascades to influence gene expression, the steroid hormones bind to intracellular receptors, which then bind to DNA and regulate gene expression directly. ER-b has a molecular mass of
approximately 53kD and is composed of six regions, A to F, on the basis of different amino acid sequence homology (2). The A/B region contains a transcription activation function. Region C encodes the DNA binding domain. Region D is involved in binding to hsp90, as well as containing nuclear localization signals and plays a part in stabilizing DNA binding by the DNA binding domain. Region E contains the hormone/ligand binding domain as well as hormone-inducible transcription activation function. Region F appears to play a role in modulating transcriptional activation by ER-a.
ER-b was discovered in 1996 in rat prostate, as well as in secretory epithelial cells of the prostate and granulose cells of the ovary (1). Later, human ER-b was discovered and characterized and found to be expressed in human thymus, spleen, ovary and testis (3).
Catalog #E3565-17
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1ug/ml
Western Blot: 1-2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.25mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenA 19 amino acid synthetic peptide derived from the C-terminus of the human Estrogen Receptor beta protein
SpecificityRecognizes human Estrogen Receptor beta protein (~53kD). Reactivity was con rmed by Western Blot using recombinant human Estrogen Receptor beta and MCF-7 cell lysate.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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