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You are here:Home » Antibodies » Abs to Enzymes, Sulfotransferase » Anti -Estrogen Sulfotransferase

Anti -Estrogen Sulfotransferase

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Mammalian EST sulfurylates the hydroxyl group of estrogenic steroids by transferring the sulfate from a cosubstrate adenosine 3'-phosphate-5'-phosphosulfate. Sulfurylated steroids do not bind to the estrogen receptor with high affinity and, therefore, are hormonally inactive. EST plays an important role in controlling the intracellular level of the receptor-active estrogens.
Catalog #E3565-18B
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.5-4ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.5% BSA, 0.01% thimerosal, 30% glycerol.
PurityPurified by affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa195 from human Estrogen Sulfotransferase (EST).
SpecificityRecognizes human Estrogen Sulfotransferase (EST) at ~31kD. Species Crossreactivity: rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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