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You are here:Home » Antibodies » Abs to Enzymes, Sulfotransferase » Anti -Estrogen Sulfotransferase (BSA & Azide Free)

Anti -Estrogen Sulfotransferase (BSA & Azide Free)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH IF
Mammalian EST sulfurylates the hydroxyl group of estrogenic steroids by transferring the sulfate from a cosubstrate adenosine 3-phosphate-5-phosphosulfate. Sulfurylated steroids do not bind to the estrogen receptor with high affinity and, therefore, are hormonally inactive. EST plays an important role in controlling the intracellular level of the receptor active estrogens.
Catalog #E3565-18X
ApplicationsSuitable for use in Immunofluorescence and Immunohistochemistry (formalin/paraffin). Other applications not tested.
Recommended DilutionImmunohistochemistry: 2-4ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min., followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive ControlRat liver
Cellular LocalizationCytoplasmic with some nuclear
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No0.N.290
HostMouse
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4.
Also available with BSA and azide, see cat# E3565-18.
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified rat liver estrogen sulfotransferase
SpecificityRecognizes rat estrogen sulfotransferase (EST) at ~31kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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