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You are here:Home » Antibodies » Abs to Cell Markers » Anti -ER-HR3

Anti -ER-HR3

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified IH FC
Mouse ER-HR3 is a cell surface antigen which is expressed by Langerhans cells in epithelium, a subset of mature macrophages and dendritic cells that are located predominantly in hematopoietic and lymphoid organs. Approximately 30% of bone
marrow cells express the antigen. Other ER-HR3 positive macrophages can be found in the splenic red pulp, in the
mesenteric lymphoid paracortex, interfollicular areas of Peyer’s patches and bone marrow. Few ER-HR3 positive
cells are observed in the thymic cortex and the connective tissues of the gastro-intestinal tract, the dermis and the
renal medulla. Epidermal Langerhans cells also express the antigen.
Catalog #E3566
ApplicationsSuitable for use in Flow cytometry and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionsFlow cytometry: 1:10-1:50
Immunohistochemistry: frozen
Optimal dilutions to be determined by the researcher.
HybridomaHybridization of P3-X63-Ag8.563 myeloma cells with spleen cells from immunized rats.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2c
Clone NoER-HR3
HostRat
SourceMouse
ConcentrationNot determined
FormSupplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
PurityPurified by Protein G chromatography.
ImmunogenAdherent F1 (CBAxBL) bone marrow stromal cells.
SpecificityRecognizes mouse ER-HR3. Recognizes two proteins of approximately 69kD and 55kD under non-reducing conditions. Does not inhibit T cell proliferation in antigen-specific T-cell proliferation studies.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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