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Specifications

Clone Host Grade Applications Monoclonal Rat Affinity Purified IH FC

Mouse ER-HR3 is a cell surface antigen which is expressed by Langerhans cells in epithelium, a subset of mature macrophages and dendritic cells that are located predominantly in hematopoietic and lymphoid organs. Approximately 30% of bone
marrow cells express the antigen. Other ER-HR3 positive macrophages can be found in the splenic red pulp, in the
mesenteric lymphoid paracortex, interfollicular areas of Peyer’s patches and bone marrow. Few ER-HR3 positive
cells are observed in the thymic cortex and the connective tissues of the gastro-intestinal tract, the dermis and the
renal medulla. Epidermal Langerhans cells also express the antigen.
Catalog #	E3566
Applications	Suitable for use in Flow cytometry and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions	Flow cytometry: 1:10-1:50
	Immunohistochemistry: frozen
	Optimal dilutions to be determined by the researcher.
Hybridoma	Hybridization of P3-X63-Ag8.563 myeloma cells with spleen cells from immunized rats.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2c
Clone No	ER-HR3
Host	Rat
Source	Mouse
Concentration	Not determined
Form	Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity	Purified by Protein G chromatography.
Immunogen	Adherent F1 (CBAxBL) bone marrow stromal cells.
Specificity	Recognizes mouse ER-HR3. Recognizes two proteins of approximately 69kD and 55kD under non-reducing conditions. Does not inhibit T cell proliferation in antigen-specific T-cell proliferation studies.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.