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You are here:Home » Antibodies » Abs to Cell Markers » Anti -ER-HR3

Anti -ER-HR3

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified IH FC
Catalog #E3566-01
ApplicationsSuitable for use in Flow Cytometry and Immunohistochemistry (frozen). Other applications not tested.
Recommended DilutionFlow Cytometry: 1:10-1:50; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.
HybridomaP3-X63-Ag8.563 myeloma cells with spleen cells from immunized rats.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2c
Clone NoER-HR3
HostRat
SourceMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenAdherent F1 (CBAxBL) bone marrow stormal cells.
SpecificityRecognizes mouse antigen ER-HR3. ER-HR3 is a cell surface antigen which is expressed by Langerhans cells in epithelium, a subset of mature macrophages and dendritic cells that are located predominantly in hematopoietic and lymphoid organs. Initial studies of ER-HR3 demonstrated expression on peripheral blood monocytes but recent studies suggest that expression is actually very low.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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