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Specifications

Clone Host Grade Applications Monoclonal Rat Affinity Purified IH FC

Catalog #	E3566-01
Applications	Suitable for use in Flow Cytometry and Immunohistochemistry (frozen). Other applications not tested.
Recommended Dilution	Flow Cytometry: 1:10-1:50; 10ul labels 10e6 cells in 100ul
	Optimal dilutions to be determined by the researcher.
Hybridoma	P3-X63-Ag8.563 myeloma cells with spleen cells from immunized rats.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2c
Clone No	ER-HR3
Host	Rat
Source	Mouse
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Adherent F1 (CBAxBL) bone marrow stormal cells.
Specificity	Recognizes mouse antigen ER-HR3. ER-HR3 is a cell surface antigen which is expressed by Langerhans cells in epithelium, a subset of mature macrophages and dendritic cells that are located predominantly in hematopoietic and lymphoid organs. Initial studies of ER-HR3 demonstrated expression on peripheral blood monocytes but recent studies suggest that expression is actually very low.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.