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You are here:Home » Molecular Biology » MB-Translation Initiation Factors » EIF1, Recombinant, Human (Eukaryotic Translation Initiation Factor 1, A121, EIF-1, EIF1A, ISO1, SUI1)

EIF1, Recombinant, Human (Eukaryotic Translation Initiation Factor 1, A121, EIF-1, EIF1A,
ISO1, SUI1)

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Specifications

EIF1, also known as eukaryotic translation initiation factor 1, is a universally conserved translation factor that is necessary for scanning and involved in initiation site selection. This protein promotes the assembly of 48S ribosomal complexes at the authentic initiation codon of a conventional capped mRNA.
Catalog #E8947-95
Recombinant human EIF1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Endotoxin 1EU/1ug (LAL).
Amino Acid SequenceMRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWAGSMSA IQNLHSFDPF ADASKGDDLL PAGTEDYIHI RIQQRNGRKT LTTVQGIADD YDKKKLVKAF KKKFACNGTV IEHPEYGEVI QLQGDQRKNI CQFLVEIGLA KDDQLKVHGF
Molecular Weight 16.9kD (150aa)
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight16.9kD
SourceE.coli
Purity~90% by SDS-PAGE.
Concentration~0.5mg/ml
FormSupplied as a liquid in 20mM Tris-HCl buffer, pH8, 1mM DTT, 0.1M NaCl, 10% glycerol.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesA121, EIF-1, EIF1A, ISO1, SUI1


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