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You are here:Home » Antibodies » Abs to Translation Initiation Factors » Anti -Eukaryotic Translation Initiation Factor 2, alpha (eIF2a)

Anti -Eukaryotic Translation Initiation Factor 2, alpha (eIF2a)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IC
EIF-2alpha, also known as eukaryotic translation initiation factor 2alpha, promotes the binding of the initiator tRNA to the 40S ribosomal subunits and controls translational rates via phosphorylation/dephosphorylation mechanisms. The double-stranded RNA (dsRNA)-activated protein kinase (PKR) provides an important control in the regulation of protein synthesis initiation through the phosphorylation of eIF-2alpha. Phosphorylated eIF-2 alpha inhibits cell growth and induces apoptosis (1). Phosphorylation of eIF-2alpha occurs under a variety of conditions including viral infection, apoptosis, nutrient deprivation, heme-deprivation, and certain stresses (2). EIF-2 exists as a heterotrimeric protein composed of 3 nonidentical subunits, alpha (36kD), beta (38kD), and gamma (52 kD) (3).
Applications:
Suitable for use in Western Blot and Immunocytochemistry. Other applications have not been tested.
Catalog #E8949-01
Recommended DilutionWestern Blot (ECL): 1ug/ml
Optimal dilutions to be determined by researcher.
Positive Controls:
HeLa Cell Lysate: Use 20ug per lane.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide 13aa of Rat eIF-2alpha phosphorylated (KLH coupled).
SpecificityRecognizes a ~36kD protein, corresponding to the apparent molecular mass of rat eIF-2alpha. Species Crossreactivity: human, mouse, bovine, canine, guinea pig, hamster, monkey, porcine, rabbit and sheep.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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