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You are here:Home » Antibodies » Abs to Translation Initiation Factors » Anti -Eukaryotic Translation Initiation Factor 2, alpha, phosphorylated (Ser51) (eIF2a)

Anti -Eukaryotic Translation Initiation Factor 2, alpha, phosphorylated (Ser51) (eIF2a)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Purified B IH IC FC IF
Eukaryotic initiation factor 2 (eIF2) plays a central role in initiating translation, and provides for a rate-limiting step in protein synthesis. Phosphorylation of a-subunit of eIF2 effectively prevents formation of the eIF2/GTP/Met-tRNAi complex and inhibits global protein synthesis. Three distinct protein kinases regulate protein synthesis in eukaryotic cells by phosphorylating the a-subunit of eIF2 at Serine51. Phosphorylation occurs under a wide variety of different stimuli, including heat shock, serum deprivation, glucose starvation, amino acid starvation, exposure to heavy metal ions, and viral infection.
Catalog #E8949-01B
ApplicationsSuitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Flow Cytometry and Dot Blot. Not recommended for use in Immunoprecipitation. Other applications not tested.
Recommended DilutionsWestern Blot: 1:500. Detects a band at ~36kD
Immunofluorescence (IC): 1:100-1:250
Immunohistochemistry: Frozen and paraffin sections
Flow Cytometry: 1:100
Dot Blot: 1:500
Optimal dilutions to be determined by the researcher.
Positive ControlPC12 cell lysate, human liver carcinoma
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No6k169
HostRabbit
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.05% BSA, 0.01% sodium azide, 50% glycerol.
PurityPurified
ImmunogenA synthetic phospho-peptide corresponding to residues surrounding Ser51 of human eIF-2a. Species Sequence Homology: bovine and porcine
SpecificityRecognizes human eIF-2a phosphorylated at serine 51. Species Crossreactivity: mouse and rat, Drosophila melanogaster, plants, African Green Monkey, Neurospora crassa
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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