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Anti -Eukaryotic Translation Initiation Factor 2, alpha (eIF2a)
Anti -Eukaryotic Translation Initiation Factor 2, alpha (eIF2a)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
B |
|
| EIF-2a (eukaryotic translation initiation factor 2 alpha subunit) is a subunit of the eIF-2 protein, an important regulator of translation initiation. Phosphorylation of eIF-2a on Ser 51 increases the affinity of eIF-2a for eIF-2B, a guanine nucleotide exchange factor needed for the recycling of eIF-2-GDP to eIF-2-GTP. Reduction of eIF-2-GTP levels leads to the suppression of the overall rate of protein synthesis. Heme-regulated inhibitor (HRI), ER-resident protein kinase PERK, dsRNA activated protein kinase PKR, and the homologue of the S. cerevisiae protein kinase GCN2 are all eIF-2a kinases. | | | | | | Applications: | | | Western Blot: An antibody concentration of 1ug/mL is recommended. | | | Catalog # | E8949-01C | | Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Goat | | Source | Human | | Concentration | As reported | | Form | Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 0.02% sodium azide. Reconstitute with sterile PBS, 40-50% glycerol. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | E. coli-derived recombinant human eIF-2a (rheIF-2a, aa1-315) | | Specificity | Detects human, mouse, and rat eIF-2a. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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