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You are here:Home » Antibodies » Abs to Translation Initiation Factors » Anti -Eukaryotic Translation Initiation Factor 2, alpha (eIF2a)

Anti -Eukaryotic Translation Initiation Factor 2, alpha (eIF2a)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified B
EIF-2a (eukaryotic translation initiation factor 2 alpha subunit) is a subunit of the eIF-2 protein, an important regulator of translation initiation. Phosphorylation of eIF-2a on Ser 51 increases the affinity of eIF-2a for eIF-2B, a guanine nucleotide exchange factor needed for the recycling of eIF-2-GDP to eIF-2-GTP. Reduction of eIF-2-GTP levels leads to the suppression of the overall rate of protein synthesis. Heme-regulated inhibitor (HRI), ER-resident protein kinase PERK, dsRNA activated protein kinase PKR, and the homologue of the S. cerevisiae protein kinase GCN2 are all eIF-2a kinases.
Applications:
Western Blot: An antibody concentration of 1ug/mL is recommended.
Catalog #E8949-01C
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder from a 0.2um filtered solution in PBS with 0.02% sodium azide. Reconstitute with sterile PBS, 40-50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenE. coli-derived recombinant human eIF-2a (rheIF-2a, aa1-315)
SpecificityDetects human, mouse, and rat eIF-2a.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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