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You are here:Home » Antibodies » Abs to Translation Initiation Factors » Anti -Eukaryotic Translation Initiation Factor 2, alpha, phosphorylated (Ser51) (eIF2a)

Anti -Eukaryotic Translation Initiation Factor 2, alpha, phosphorylated (Ser51) (eIF2a)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH
Phosphorylation of the eukaryotic initiation factor 2 (eIF2) alpha subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. Eukaryotic initiation factor 2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2). eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B (1,2). Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2) or heme deficiency (HRI) can phosphorylate the alpha subunit of eIF2 (3,4). This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-gamma and TNF-alpha induces potent phosphorylation of eIF2alpha at Ser51 (5,6)
Catalog #E8949-03
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions
Western Blot: 1:1000
Immunoprecipitation: 1:100
Immunohistochemistry (Paraffin): 1:50 IHC protocol: Unmasking buffer/Antibody diluent
Citrate/TBST-5%NGS
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No9B148
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol, 0.02% sodium azide.
PurityAscites
ImmunogenSynthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser51 of human eIF2alpha.
SpecificityRecognizes endogenous human eIF2 alpha only when phosphorylated at Ser51. The antibody does not recognize elF2alpha phosphorylated at other sites. Species crossreactivity: mouse, rat, monkey and D. melanogaster.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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