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You are here:Home » Antibodies » Abs to Translation Initiation Factors » Anti -Eukaryotic Translation Initiation Factor 2, alpha, phosphorylated (Ser52) (eIF2a)

Anti -Eukaryotic Translation Initiation Factor 2, alpha, phosphorylated (Ser52) (eIF2a)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
eIF2a is a 36kD protein which is ubiquitously expressed in many cell types. The eIF2 protein, which is composed of three subunits (a, b and g), is one of the key molecules in the initiation of translation. In mammalian cells, stress induces eIF2a to be phosphorylated at serine 52 by at least two kinases: the haem-controlled repressor (HCR) and the interferon inducible double stranded RNA-dependent protein kinase (PKR). Phosphorylation of eIF2a blocks the GDP-GTP exchange activity of eIF2b, leading to inhibition of protein synthesis, growth suppression, and apoptosis induction. The phosphorylation of eIF2a is an important regulatory process in protein synthesis.
Catalog #E8949-09
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Optimal dilution determined by the researcher.
Positive Control3T3-L1 adipocytes +/- Leukemia Inhibitory Factor (LIF).
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in Dulbecco’s PBS (without Mg2+ and Ca2+), pH 7.3, 1mg/ml BSA (IgG, protease free), 0.05% sodium azide, 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic phosphopeptide derived from the region of human eIF2a that contains serine 52.
SpecificityRecognizes human eIF2a when phosphorylated at Ser52. Species Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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