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You are here:Home » Antibodies » Abs to Translation Initiation Factors » Anti -Eukaryotic Translation Initiation Factor 2, alpha (Eukaryotic Tanslation Initiation Factor 2 Subunit alpha, eIF-2alpha, eIF-2-alpha, eIF2a, eIF-2A, EIF2, EIF-2, Eukaryotic Translation Initiation Factor 2 Subunit 1, EIF2S1)

Anti -Eukaryotic Translation Initiation Factor 2, alpha (Eukaryotic Tanslation Initiation
Factor 2 Subunit alpha, eIF-2alpha, eIF-2-alpha, eIF2a, eIF-2A, EIF2, EIF-2, Eukaryotic
Translation Initiation Factor 2 Subunit 1, EIF2S1)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Purified B IP IH
Phosphorylation of the eukaryotic initiation factor 2 (eIF2) a subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. Eukaryotic initiation factor 2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to
form the 43S preinitiation complex (1,2). eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B (1,2). Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK),aa deprivation (GCN2) or heme deficiency (HRI) can phosphorylate the a subunit of eIF2 (3,4). This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of
PKR by IFN-y and TNF-a induces potent phosphorylation of eIF2a at Ser51 (5,6).
Catalog #E8949-09E
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Immunohistochemistry (Pafarrin):1:800
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG
Clone No11C1031
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, less than 0.02% sodium azide, 50% glycerol.
PurityPurified
ImmunogenRecombinant human eIF2a.
SpecificityRecognizes endogenous levels of total eIF2a. Species Crossreactivity: monkey, mouse, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesEukaryotic Translation Initiation Factor 2, Subunit 1 alpha, 35kDa


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