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You are here:Home » Antibodies » Abs to Translation Initiation Factors » Anti -EIF2AK4 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 4, GCN2-like Protein, GCN2, KIAA1338)

Anti -EIF2AK4 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 4, GCN2-like
Protein, GCN2, KIAA1338)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B
GCN2, also known as EIF2AK4, is a widely expressed 190kD ribosome-associated Ser/Thr kinase that plays an important role in the control of protein translation. In response to aa deprivation or UV irradiation, GCN2 phosphorylates the alpha subunit of eukaryotic initiation factor 2 (eIF2a) at Ser51 and induces a delay in entry to S phase of the cell cycle. Increased GCN2 activity in solid tumors enhances the production of aa's and contributes to tumor cell survival in conditions of nutrient deprivation. GCN2 contains an RWD domain aa21-134, a pseudo-kinase domain aa296-539, a catalytic domain aa590-1001, and a histidyl tRNA synthase-like domain aa1022-1493 followed by a C-terminal ribosome interacting region. Alternate splicing generates an isoform with a short deletion in the kinase domain and an isoform that is truncated at the N-terminal end of the kinase domain. Within aa22-139, human GCN2 shares 91% aa sequence identity with mouse and rat GCN2.
Catalog #E8949-18F
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2B
Clone No12K94
HostMouse
SourceHuman
Concentration~0.5mg/ml after reconstitution
FormSupplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.
PurityPurified by Protein A/G affinity chromatography.
ImmunogenRecombinant corresponding to aa22-139 from human GCN2 expressed in E.coli.
SpecificityRecognizes human GCN2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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