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You are here:Home » Antibodies » Antibodies-Translation Initiation Factors » Anti -Eukaryotic Translation Initiation Factor 2B, epsilon, phosphorylated (Ser539) (eIF2Be)

Anti -Eukaryotic Translation Initiation Factor 2B, epsilon, phosphorylated (Ser539)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Eukaryotic Initiation Factor 2B (eIF2B) plays a key role in the initiation of mRNA translation in eukaryotic cells by acting as a guanine nucleotide exchange factor. The activity of eIF2B is regulated by a wide array of conditions. Stress inhibits its action, while stimulation of protein synthesis results in its activation. eIF2B , an 82kD subunit of eIF2B, is phosphorylated at serine 539 by glycogen synthase kinase-3 (GSK-3), which leads to inhibition of its guanine nucleotide exchange activity.
Catalog #E8949-21B
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: 1:1000.
Optimal dilutions to be determined by the researcher.
Recommended Positive ControlsRecombinant eIF2B treated with GSK-3b; CHO-T cells transfected with human insulin receptor (IR) +/- insulin.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
ConcentrationNot determined
FormSupplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.
PurityPurified by affinity chromatography.
ImmunogenSynthetic phosphopeptide derived from the region of rat eIF2B that contains serine Ser535 (corresponding to serine 539 in the human sequence).
SpecificityRecognizes rat eIF2B when phosphorylated at Ser539. Species sequence homology: human (80%).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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