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You are here:Home » Antibodies » Abs to Translation Initiation Factors » Anti -EIF3E (Eukaryotic Translation Initiation Factor 3 Subunit E, eIF3e, Eukaryotic Translation Initiation Factor 3 Subunit 6, Viral Integration Site Protein INT-6 Homolog, eIF-3 p48, EIF3S6, INT6)

Anti -EIF3E (Eukaryotic Translation Initiation Factor 3 Subunit E, eIF3e, Eukaryotic
Translation Initiation Factor 3 Subunit 6, Viral Integration Site Protein INT-6 Homolog,
eIF-3 p48, EIF3S6, INT6)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins.
Catalog #E8949-32
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:2000
Western Blot: 0.03-0.1ug/ml, lysates of cell line Daudi observed on ~50kD bands
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to C-KLNQNSRSEAPN, from human EIF3E, at C-terminal of the protein (NP_001559.1).
SpecificityRecognizes human EIF3E. Species sequence homology: Bovine, canine and porcine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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