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You are here:Home » Molecular Biology » MB-Translation Initiation Factors » EIF3S1, Recombinant, Human, aa70-258, His-tag (Eukaryotic Translation Initiation Factor 3 Subunit J, eIF3j, Eukaryotic Translation Initiation Factor 3 Subunit 1, eIF-3-alpha, eIF3 p35, EIF3J, PRO0391)

EIF3S1, Recombinant, Human, aa70-258, His-tag (Eukaryotic Translation Initiation Factor 3
Subunit J, eIF3j, Eukaryotic Translation Initiation Factor 3 Subunit 1, eIF-3-alpha, eIF3
p35, EIF3J, PRO0391)

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Specifications

EIF3J (Eukaryotic translation initiation factor 3 subunit J) belongs to the EIF-3 subunit J family. EIF3 plays a central role in binding of initiator methionyl-tRNA and mRNA to the 40S ribosomal subunit to form the 40S initiation complex. EIF3J binds to the aminoacyl (A) site and mRNA entry channel of the 40S subunit, placing EIF3J directly in the ribosomal decoding center. EIF3J also interacts with eIF1A and reduces 40S subunit affinity for mRNA. A high affinity for mRNA is restored upon recruitment of initiator tRNA, even though EIF3J remains in the mRNA-binding cleft in the presence of tRNA.
Catalog #E8949-34C
SourceRecombinant corresponding to aa70-258 from human EIF3J, fused to His-tag at N-terminal expressed in E.coli.
AA SequenceMGSSHHHHHH SSGLVPRGSH MKISEKKKIA EKIKEKERQQ KKRQEEIKKR LEEPEEPKVL TPEEQLADKL RLKKLQEESD LELAKETFGV NNAVYGIDAM NPSSRDDFTE FGKLLKDKIT QYEKSLYYAS FLEVLVRDVC ISLEIDDLKK ITNSLTVLCS EKQKQEKQSK AKKKKKGVVP GGGLKATMKD DLADYGGYDG GYVQDYEDFM
Molecular Weight~24kD (MALDI-TOF)
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~24kD
SourceE.coli
Purity~90% (SDS-PAGE)
Concentration~0.5mg/ml
FormSupplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 2mM DTT, 10% glycerol, 200mM sodium chloride.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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