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E8949-38 Rabbit Anti-Eukaryotic Translation Initiation Factor 4A (eIF4A)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
P60842, Q14240, P38919
Isotype
IgG
Grade
Affinity Purified
Applications
WB
Crossreactivity
Hu Mk Mo Rt
Gene ID
1973, 1974, 9775
Shipping Temp
Blue Ice
Storage Temp
-20°C

A variety of factors contribute to the important biological event of initiation of translation. The eIF4F complex of translation initiation factors binds to the 5' m7 GTP cap to open up the mRNA secondary structure and allow small ribosome subunit binding (1). eIF4A, an eIF4 complex component that acts as an ATP-dependent RNA helicase, unwinds the secondary structure of the 5' mRNA untranslated region to mediate ribosome binding (2,3). In addition, eIF4A has recently been shown to repress Dpp/BMP signalling in a translation-independent manner in Drosophila (4,5).

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide derived from residues surrounding Phe52 of human eIF4A
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Detects endogenous levels of human total eIF4A1, eIF4A2 and eIF4A3 proteins. Species Crossreactivity: mouse, monkey and rat
References
1. Rogers, G.W. et al. (2001) J. Biol. Chem. 276:12598-12608. 2. Rogers, G.W. et al. (1999) J. Biol. Chem. 274:12236-12244. 3. Svitkin, Y.V. et al. (2001) RNA 7:382-394. 4. Li, J. and Li, W.X. (2006) Nat. Cell Biol. 8:1407-1414. 5. Affolter, M. and Pyrowolakis, G. (2006) Nat. Cell Biol. 8:1319-1321.
USBio References
No references available
Conjugates
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