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You are here:Home » Antibodies » Abs to Translation Initiation Factors » Anti -Eukaryotic Translation Initiation Factor 4B (eIF4B)

Anti -Eukaryotic Translation Initiation Factor 4B (eIF4B)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IH IC FC
Eukaryotic protein synthesis initiation factor 4B (eIF-4B) is an 80,000 dalton polypeptide which is essential for the binding of mRNA to ribosomes.The amino-terminal domain of eIF-4B contains a consensus RNA binding domain present in a number of other RNA binding proteins (1).It contains a ribonucleoprotein consensus sequence (RNP- CS)/RNA recognition motif (RRM). Deletions which remove this region abolish the ability of eIF-4B to cooperate with eIF-
4A in RNA binding and the ability to stimulate the helicase activity of eIF-4A. Results indicate that the carboxy-terminal RNA-binding region of eIF-4B is essential for eIF-4B function and is distinct from the RNP-CS/RRM (2). eIF4F binds to the mRNA cap structure and, in combination with eIF4B, is believed to unwind the secondary structure in the 5' untranslated region to facilitate ribosome binding. It has been suggested that eIF4B participates in mRNA-ribosome binding
by acting as an intermediary between the mRNA and eIF3, via a direct interaction with the p170 subunit of eIF3 (3).
Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Catalog #E8949-39E
Recommended DilutionFlow Cytometry: 1:20
Western Blot: 1:200,000
Immunohistochemistry: 1:100-1:250
Immunocytochemistry: 1:100-1:250
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No9H348
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
PuritySupernatant
ImmunogenA synthetic peptide corresponding to residues on the C-terminus of human Eif4b.
SpecificityRecognizes human Eif4b.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate nameseIF 4B, eIF-4B


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