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You are here:Home » Antibodies » Abs to Translation Initiation Factors » Anti -Eukaryotic Translation Initiation Factor 4E , phosphorylated (Ser209) (eIF4E, eIF4EL1)

Anti -Eukaryotic Translation Initiation Factor 4E , phosphorylated (Ser209) (eIF4E,
eIF4EL1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH IF
Catalog #E8949-40E
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and Immunofluorescence. Other applications not tested.
Recommended DilutionWestern Blot: 1:500-1:1000
Immunohistochemistry (paraffin): 1:50-1:100
Immunofluorescence: 1:100-1:200
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic phosphopeptide derived from human eIF4E around the phosphorylation site of serine 209 (S-G-SP-T-T).
SpecificityRecognizes endogenous levels of human elF4E at ~25kD only when phosphorylated at serine 209. Species Crossreactivity: mouse, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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