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You are here:Home » Antibodies » Abs to Translation Initiation Factors » Anti -Eukaryotic Translation Initiation Factor 4E, phosphorylated (Ser209) (eIF4E, eIF4EL1)

Anti -Eukaryotic Translation Initiation Factor 4E, phosphorylated (Ser209) (eIF4E,
eIF4EL1)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH IC
Catalog #E8949-40F
eIF4E is a eukaryotic translation initiation factor that is involved in the direction of ribosomes to the cap structure of mRNA's. There are two forms of eIF4E a free form (25kD) and as part of a multi-protein complex (eIF-4F). The free form seems to not be as plentiful, compared to the other initiation factors and acts as a rate limiting step of initiation.
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry (paraffin), and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:5000-10000
Immunohistochemistry (paraffin): 1:100-250
Immunocytochemistry: 1:500
Immunoprecipitation: 1:60
Optimal dilutions to be determined by the researcher.
Positive Control293 cell lysate
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No9i156
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
PuritySupernatant
ImmunogenSynthetic peptide corresponding to residues surrounding phosphorylated Ser209 of human eIF4E.
SpecificityRecognizes human eIF4E phosphorylated at Ser209. Species Crossreactivity: mouse, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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