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You are here:Home » Antibodies » Abs to Translation Initiation Factors » Anti -Eukaryotic Translation Initiation Factor 4E (eIF-4E, eIF4E, EIF4E1, CBP, EIF4EL1, EIF4F, eIF-4F 25 kD Subunit, MGC111573, mRNA Cap-binding Protein)

Anti -Eukaryotic Translation Initiation Factor 4E (eIF-4E, eIF4E, EIF4E1, CBP, EIF4EL1,
EIF4F, eIF-4F 25 kD Subunit, MGC111573, mRNA Cap-binding Protein)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
eIF4E is the smallest subunit of eIF4F, which is a supramolecular complex consisting of three subunits, eIF4E, eIF4A, and eIF4G, and catalysing the initiation of cap-dependent translation with a sequential function. eIF4E binds selectively to the cap structure and is a master switch that controls eukaryotic translation, because the binding of eIF4E to the cap structure is required for initiating the translation of mRNA and is regulated through the association/dissociation of eIF4E with endogenous 4E-binding proteins (4E-BPs). The protein also promotes cellular growth and transformation. The levels of eIF4E are used as prognostic indicators of clinical outcome in human cancers, including breast cancer and head and neck squamous cell carcinomas. Recent studies reveal that eIF4E also acts as a promoter of nucleocytoplasmic transport of specific transcripts
Catalog #E8949-40T
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:500-1:1000
Immunohistochemistry (paraffin): 1:50-1:100
Optimal dilutions to be determined by the researcher.
Positive ControlNIH/3T3 cells
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic non-phosphopeptide corresponding to human elF4E around the phosphorylation site of serine 209 (S-G-SP-T-T).
SpecificityRecognizes human eIF4E. Species crossreactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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