EIF4E2, NT

Clone Type

Polyclonal

Host

Rabbit

Source

Human

Isotype

IgG

Grade

Purified

Applications

E IHC WB

Crossreactivity

Accession #

NP_004837; O60573

Shipping Temp

Blue Ice

Storage Temp

-20°C

EIF4E2 is expressed exclusively in the cytoplasm. This protein recognizes and binds the 7 methylguanosine containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.

Applications

Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution

ELISA: 1:1,000 Western Blot: 1:100-1:500 Immunohistochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

Synthetic peptide selected from the N-terminal region of human EIF4E2 (KLH).

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Purity

Purified by ammonium sulfate precipitation

Specificity

Recognizes human EIF4E2.

References

1. Rom, E., et al., J. Biol. Chem. 273(21):13104-13109 (1998). 2. Mao, M., et al., Proc. Natl. Acad. Sci. USA 95(14):8175-8180 (1998). 3. Tee, A.R., et al., FEBS Lett. 564(1-2), 58-62 (2004).