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You are here:Home » Antibodies » Abs to Translation Initiation Factors » Anti -Eukaryotic Translation Initiation Factor 5A, CT (eIF 5A, eIF5A Protein Synthesis Initiation Factor)

Anti -Eukaryotic Translation Initiation Factor 5A, CT (eIF 5A, eIF5A Protein Synthesis
Initiation Factor)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH IC FC
The human eukaryotic translation initiation factor 5A (eIF-5A) is an 18kD protein. eIF-5A precursor [(ec-eIF-5A(lys)] is the only cellular protein known to contain a specific lysine residue which is transformed into the unique amino acid hypusine [N-(4-amino-2-hydroxybutyl)-lysine] by a series of post-translational reactions. eIF-5A promotes the formation of the first peptide bond during the initial stage of protein synthesis.
Catalog #E8950-05C
ApplicationsSuitable for use in Flow Cytometry, ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionsFlow Cytometry: 1:20
Western Blot: 1:1000
Immunohistochemistry: 1:100-1:250
Immunocytochemistry: 1:100-1:250
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride. 0.05% BSA, 0.01% sodium azide, 40% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenA synthetic peptide corresponding to residues close to the C-terminus of human eIF-5A.
SpecificityRecognizes human eIF-5A at ~18kD. Species Crossreactivity: mouse and rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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