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You are here:Home » Molecular Biology » MB-Binding Proteins » Ewing Sarcoma Breakpoint Region 1 (EWS, EWSR1, CAGF29) (Control Peptide)

Ewing Sarcoma Breakpoint Region 1 (EWS, EWSR1, CAGF29) (Control Peptide)

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Specifications

C-TSYDQSSYSQQNTYG, from the internal region of human EWS.
Control peptide corresponding to polyclonal antibody, Catalog#E9002-85F, goat x human.
Catalog #E9002-85F-P
This gene encodes a putative RNA binding protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in two products. Might normally function as a repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.
ApplicationsSuitable for use in ELISA. Other applications not tested.
Recomended DilutionsOptimal dilutions to be determined by the researcher
Positive ControlNuclear lysates
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 monthsat -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Peptide Blocking (see corresponding antibody E9002-85F)Antibodies are typically supplied at 0.5mg/ml and peptides as a 100ul pellet. When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
PurityHighly Purified
Concentration~0.5mg/ml
FormSupplied as a lyophilized powder in PBS.
SpecificityRecognizes human EWS at ~90kD. Species sequence homology: mouse, rat, bovine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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