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Exodus 2, Recombinant, Human (SLC, Secondary Lymphoid-tissue Chemokine, 6Ckine, TCA4)
Exodus 2, Recombinant, Human (SLC, Secondary Lymphoid-tissue Chemokine, 6Ckine, TCA4)
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| SLC/CCL21, normally expressed in high endothelial venules and in T cell zones of spleen and lymph nodes, strongly attracts T cells and dendritic cells (DC). Secondary lymphoid tissue chemokine (CCL21/SLC) regulates the homing of naive T cells and dendritic cells that express CC-chemokine receptor 7 (CCR7) from distant sites to lymphoid tissue such as lymph nodes. | | | Catalog # | E9005-08H | | Description | Recombinant Human Exodus-2 is also known as SLC (Secondary Lymphoid-Tissue Chemokine), TCA-4 (T-cell activation-4) , and 6Ckine. Recombinant CCL21 produced in E.Coli as a single, non-glycosylated, polypeptide chain containing 111 amino acids and having a molecular mass of 12219 Dalton. Recombinant CCL-21 is purified by proprietary chromatographic techniques. | | Amino Acid Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be, Ser-Asp-Gly-Gly-Ala. | | Dimers and Aggregates | Less than 1% as determined by silver-stained SDS-PAGE gel analysis. | | Biological Activity | Recombinant Human Exodus-2 is fully biologically active when compared to standard. The activity is determined by the chemoattract of human PBE (peripheral blood eosinophils) at a concentration between 0.1-10ng/ml. | | Endotoxin | 0.1ng/ug (IEU/ug) of Exodus-2. | | Protein Content | Protein quantitation was carried out by two independent methods: | | 1. UV spectroscopy at 280 nm. | | 2. Analysis by RP-HPLC, using a calibrated solution of Exodus-2 as a Reference Standard. | | Solubility | It is recommended to reconstitute the lyophilized Exodus-2 in sterile 18M -cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | | Stability | Lyophilized Exodus-2 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Exodus-2 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. | | Molecular Weight | 12.22kD | | Source | E. coli | | Purity | 98% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel. | | Concentration | ~1mg/ml | | Form | Supplied as a lyophilized powder without additives. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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