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You are here:Home » Antibodies » Abs to Enzymes, Exonuclease » Anti -Exonuclease I (Exonuclease-I, ExoI, Exonuclease 1, Exonuclease-1, Exo1, HEX1, hExo1, hExoI, Rad2 Nuclease Family Member Homolog of S. cerevisiae Exonuclease 1)

Anti -Exonuclease I (Exonuclease-I, ExoI, Exonuclease 1, Exonuclease-1, Exo1, HEX1, hExo1,
hExoI, Rad2 Nuclease Family Member Homolog of S. cerevisiae Exonuclease 1)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
This gene encodes a protein with 5' to 3' exonuclease activity as well as an RNase H activity. It is similar to the Saccharomyces cerevisiae protein Exo1 which interacts with Msh2 and which is involved in mismatch repair and recombination. Alternative splicing of this gene results in three transcript variants encoding two different isoforms. [provided by RefSeq]
Epitope: C-HRNYSPRPESGT
Theoretical Molecular Weight: 89.1kD
Applications:
Suitable for use in Peptide ELISA and Western Blot. Other applications not tested.
Catalog #E9007-85D
Recommended DilutionPeptide ELISA: Antibody detection limit dilution 1:64,000
Western Blot: 0.2-0.5ug/ml; Approx 90kDa band observed in lysates in cell lines HEK293 and A549 (calculated MW of 89.1kDa according to NP_003677.3).
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 3 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeGG
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris-saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenPeptide with sequence C-HRNYSPRPESGT, from the internal region of the protein sequence according to NP_003677.3; NP_006018.3; NP_569082.1.
SpecificityRecognizes human Exonuclease 1. Expected to recognize all three reported isoforms (NP_003677.3; NP_006018.3 and NP_569082.1).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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