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You are here:Home » Molecular Biology » Cloning-Vectors » Baculovirus Expression Vector pAc-k-CH3

Baculovirus Expression Vector pAc-k-CH3

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Specifications

Baculovirus cassette vector pAc-k-CH3 for the expression of human, humanized or chimeric IgG(kappa) in insect cells and secretion of assembled antibodies into the supernatant. The vector derived from pAcUW51 is suitable for cloning of heavy and light chain Fv antibody gene fragments.
Catalog #E9073-50
Vector Size7363bp
ResistanceVector contains an ampicillin resistance marker gene
ApplicationsCloning of heavy and light chain variable domain genes of scFv antibody fragments selected from hybridomas, single B-lymphocytes or phage display libraries.
Cloning sitesXhol and Nhel for heavy chain VH genes.
Sacl and HindIII for light chain VL(k) genes.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PurityPurified using a Plasmid Purification Kit.
Concentration~0.5mg/ml
FormSupplied as a liquid in TE buffer.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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