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You are here:Home » Molecular Biology » Cloning-Vectors » Baculovirus Expression Vector pAc-l-Fc

Baculovirus Expression Vector pAc-l-Fc

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Specifications

Baculovirus cassette vector pAc-l-Fc for the expression of human, humanized or chimeric IgG(lambda) in insect cells and secretion of assembled antibodies into the supernatant. The vector derived from pAcUW51 is suitable for cloning of heavy and light chain Fab antibody gene fragments.
Catalog #E9073-56
Vector Size6701bp
ResistanceVector contains an ampicillin resistance marker gene
ApplicationsSuitable for use in cloning of heavy and light chain variable domain genes of scFv or Fab antibody fragments selected from hybridomas, single B-lymphocytes or phage display libraries.
Cloning sitesXhoI and SpeI for heavy chain Fab fd genes SacI and EcoRV for light chain Fab(l) genes. Expression of immunoglobulin heavy and light chains in insect cells and secretion of assembled IgG(l) antibodies into the supernatant.
Storage and StabilityFor long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PurityPurified using a Plasmid Purification Kit.
Concentration~0.5mg/ml
FormSupplied as a liquid in TE buffer.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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