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Anti -Ezrin, phosphorylated (Thr567) Radixin (Thr564) Moesin (Thr558) (Cytovillin)
Anti -Ezrin, phosphorylated (Thr567) Radixin (Thr564) Moesin (Thr558) (Cytovillin)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B IH |
|
| ERM (Ezrin-Radixin-Moesin) proteins function as plasma membrane-actin cytoskeleton linkers and participate in the formation of specialized domains of the plasma membrane. One function of ezrin is in determining survival of epithelia cells by activating the P1-3-kinase/Akt pathway. | | | Catalog # | E9100 | | Applications | Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. | | Immunohistochemistry: 1:50 paraffin | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid in 10mM HEPES, pH 7.5, 150 mM sodium chloride, 100ug/ml BSA, 50% glycerol. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Thr567 of human ezrin (KLH). | | Specificity | Recognizes Thr567 phosphorylated ezrin, Thr564 phosphorylated radixin and Thr558 phosphorylated moesin. Antibody does not cross-react with other related phospho proteins such as merlin or band 4.1. Species Crossreactivity: Human, monkey, mouse and rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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