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Anti -Ezrin (Radixin, Moesin, Cytovillin, p81, Membrane-organizing extension spike protein)
Anti -Ezrin (Radixin, Moesin, Cytovillin, p81, Membrane-organizing extension spike
protein)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B IH |
|
| ERM (Ezrin-Radixin-Moesin) proteins function as plasma membrane-actin cytoskeleton linkers and participate in the formation of specialized domains of the plasma membrane. One function of ezrin is in determining survival of epithelia cells by activating the P1-3-kinase/Akt pathway. | | | Catalog # | E9100-01B | | Applications | Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 0.1-1ug/ml detected Ezrin in RIPA lysates from A431 cells and a mourine brain lysate. | | Immunohistochemistry: 10ug/ul detects Ezrin in paraf n embedded human skin tissue sections. Optimal dilutions to be determined by the researcher. | | Positive Control Included | A0001-50: A431 Cell Lysate, Non-Stimulated. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~1mg/ml. | | Form | Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Synthetic peptide corresponding to amino acids 479-498 of human Ezrin (C-EPVSYHVQESLQDEGAEPTG). KLH-conjugated. | | Specificity | Recognizes human Ezrin, Mr 81kD. Species Crossreactivity: Mouse. Predicted to cross-react with rat based on sequence homology. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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