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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -Ezrin (Radixin, Moesin, Cytovillin, p81, Membrane-organizing extension spike protein)

Anti -Ezrin (Radixin, Moesin, Cytovillin, p81, Membrane-organizing extension spike
protein)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
ERM (Ezrin-Radixin-Moesin) proteins function as plasma membrane-actin cytoskeleton linkers and participate in the formation of specialized domains of the plasma membrane. One function of ezrin is in determining survival of epithelia cells by activating the P1-3-kinase/Akt pathway.
Catalog #E9100-01B
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 0.1-1ug/ml detected Ezrin in RIPA lysates from A431 cells and a mourine brain lysate.
Immunohistochemistry: 10ug/ul detects Ezrin in paraf n embedded human skin tissue sections. Optimal dilutions to be determined by the researcher.
Positive Control IncludedA0001-50: A431 Cell Lysate, Non-Stimulated. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml.
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to amino acids 479-498 of human Ezrin (C-EPVSYHVQESLQDEGAEPTG). KLH-conjugated.
SpecificityRecognizes human Ezrin, Mr 81kD. Species Crossreactivity: Mouse. Predicted to cross-react with rat based on sequence homology.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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