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Anti -Ezrin (Radixin, Moesin, Cytovillin, p81, Membrane-organizing extension spike protein)
Anti -Ezrin (Radixin, Moesin, Cytovillin, p81, Membrane-organizing extension spike
protein)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Purified |
B IH |
|
| Ezrin serves as a tyrosine kinase substrate and is phosphorylated in EGF-stimulated cells. | | | It is a cytoplasmic protein enriched in microvilli and other cell surfaces. Ezrin has an actin-binding capacity and is expressed in epithelial cells but not in mesenchymal cells. It is also expressed by certain epithelial tumors, such as renal cell adenocarcinomas. | | | Catalog # | E9100-20 | | Applications | Suitable for use in Immunohistochemistry (Formalin-fixed paraffin-embedded sections and Frozen sections) and Western Blot. Other applications not tested. | | Recommended Dilution | Immunohistochemistry: 1:50-1:100 for 30mins at RT (ABC method). For formalin fixed paraffin embedded sections, perform heat mediated antigen retrieval using 10mM citrate buffer pH 6.0 before commencing with Immunohistochemistry staining. | | Optimal dilutions to be determined by the researcher. | | Positive Control | | | Lung | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 4i251 | | Host | Mouse | | Source | Human | | Concentration | As reported | | Form | Supplied as a liquid in PBS with sodium azide. | | Purity | IgG fraction. | | Immunogen | Recombinant fragment, corresponding to amino acids 362-585 of Human Ezrin. | | | Specificity | Detects a protein of 81kD corresponding to human Ezrin. Species Crossreactivity: Rat, Mouse, Bovine, Hamster, Monkey and Kangaroo. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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