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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -Ezrin (Radixin, Moesin, Cytovillin, p81, Membrane-organizing extension spike protein)

Anti -Ezrin (Radixin, Moesin, Cytovillin, p81, Membrane-organizing extension spike
protein)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IH
Ezrin (also known as p81, 80K, or cytovillin) is a cytoplasmic protein enriched in microvilli and other cell surface structures. Its NH2- terminal domain is associated with the plasma membrane and the COOH-terminal domain with the cytoskeleton. Ezrin has an actin-binding capability. Ezrin is expressed in epithelial cells but not in mesenchymal cells. Ezrin is also expressed by certain epithelial tumors, such as renal cell adenocarcinomas. Ezrin serves as a tyrosine kinase substrate, and is phosphorylated in EGF-stimulated cells.
Catalog #E9100-21
Comments Excellent for staining of formalin/paraffin tissues.
Epitope aa362-585
ApplicationsImmunoprecipitation: (Native and denatured) (Use Protein G) (Ab 2ug/mg protein lysate)
Western Blot: (0.5-1ug/ml for 2hrs at RT) (1,2)
Immunohistology: (Formalin/paraffin) (1) (Use Ab 1-2ug/ml for 30 min at RT) Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Positive Control Raji cells. Lung.
Cellular Localization Cell membrane.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No5G110
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA and 0.09% sodium azide. Also available without BSA and azide at 1mg/ml.
PurityPurified by Protein G affinity chromatography.
ImmunogenA recombinant fragment of human ezrin corresponding to aa362-585.
SpecificityRecognizes Ezrin. Species Crossreactivity: Human, Monkey, Bovine, Kangaroo, Rat, Mouse, and Hamster.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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