Anti -Ezrin (Radixin, Moesin, Cytovillin, p81, Membrane-organizing extension spike
protein)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B IP IH |
|
| Ezrin (also known as p81, 80K, or cytovillin) is a cytoplasmic protein enriched in microvilli and other cell surface structures. Its NH2- terminal domain is associated with the plasma membrane and the COOH-terminal domain with the cytoskeleton. Ezrin has an actin-binding capability. Ezrin is expressed in epithelial cells but not in mesenchymal cells. Ezrin is also expressed by certain epithelial tumors, such as renal cell adenocarcinomas. Ezrin serves as a tyrosine kinase substrate, and is phosphorylated in EGF-stimulated cells. | | | Catalog # | E9100-21 | | Comments | Excellent for staining of formalin/paraffin tissues. | | Epitope | aa362-585 | | Applications | Immunoprecipitation: (Native and denatured) (Use Protein G) (Ab 2ug/mg protein lysate) | | Western Blot: (0.5-1ug/ml for 2hrs at RT) (1,2) | | Immunohistology: (Formalin/paraffin) (1) (Use Ab 1-2ug/ml for 30 min at RT) Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. | | Positive Control | Raji cells. Lung. | | Cellular Localization | Cell membrane. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 5G110 | | Host | Mouse | | Source | Human | | Concentration | ~0.2mg/ml | | Form | Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA and 0.09% sodium azide. Also available without BSA and azide at 1mg/ml. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | A recombinant fragment of human ezrin corresponding to aa362-585. | | Specificity | Recognizes Ezrin. Species Crossreactivity: Human, Monkey, Bovine, Kangaroo, Rat, Mouse, and Hamster. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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