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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -Ezrin (Radixin, Moesin, Cytovillin, p81, Membrane-organizing extension spike protein)

Anti -Ezrin (Radixin, Moesin, Cytovillin, p81, Membrane-organizing extension spike
protein)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH IC IF
Catalog #E9100-25
ApplicationsSuitable for use in Western Blotting, Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern blotting: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Immunohistochemistry (Paraffin): 1:50
Immunocytochemistry: 1:100. Immunofluorescence
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (30-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Numbern/a
Clone TypePolyclonal
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in 10mM HEPES, pH 7.5, 150 mM sodium chloride, 100ug/ml BSA, 50% glycerol.
PurityPurified by Protein A affinity chromatography.
ImmunogenKLH-conjugated, synthetic peptide corresponding to residues surrounding Thr567 of human ezrin.
SpecificityRecognizes Ezrin/Radixin/Moesin. These proteins function as linkers between the plasma membrane and the actin cytoskeleton and are involved in cell adhesion, membrane ruffling and microvilli formation. This antibody does not cross-react with other related proteins such as merlin or band 4.1. Species Crossreactivity: Human, monkey, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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