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You are here:Home » Antibodies » Abs to Carbohydrates, Glycoproteins » Anti -EMR1 (EGF-like Module Containing Mucin-like Hormone Receptor-like 1, EMR1 Hormone Receptor, Cell Surface Glycoprotein EMR1, Cell Surface Glycoprotein F4/80, DD7A5-7, EGF-TM7, F4/80, Gpf480, Lymphocyte Antigen 71, Ly71, TM7LN3) (APC)

Anti -EMR1 (EGF-like Module Containing Mucin-like Hormone Receptor-like 1, EMR1 Hormone
Receptor, Cell Surface Glycoprotein EMR1, Cell Surface Glycoprotein F4/80, DD7A5-7,
EGF-TM7, F4/80, Gpf480, Lymphocyte Antigen 71, Ly71, TM7LN3) (APC)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified FC
F4/80 antigen is a member of the EGF-TM7 family of proteins which shares 68% overall amino acid identity with human EGF module-containing mucin-like hormone receptor 1 (EMR1).
Expression of F4/80 is heterogenous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells, but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8 positive regulatory cells.
Catalog #F0001-50J
ApplicationsSuitable for use in Flow Cytometry. Other applications not tested.
Recommended DilutionsFlow Cytometry: Neat-1:10. Use 10ul labels 10e6 cells in 100ul.
Optimal dilutions to be determined by the researcher.
HybridomaSpleen cells from immunized HOB2 rats were fused with cells of the mouse NS-1 mouse myeloma cell line.
Storage and StabilityMay be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. APC is light sensitive.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No9E38
HostRat
SourceMouse
ConcentrationNot Determined
FormSupplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide, 5% sucrose. Labeled with Allophycocyanin (APC).
PurityPurified by Protein A affinity chromatography.
ImmunogenThioglycollate stimulated peritoneal cavity macrophages from C57/BL mice.
SpecificityRecognizes the mouse F4/80 antigen, a 160 kD cell surface glycoprotein that is a member of the EGF-TM7 family of proteins which shares 68% overall amino acid identity with human EGF module-containing mucin-like hormone receptor 1 (EMR1). Clone 9E38 is reported to modulate cytokine levels released in response to Listeria monocytogenes.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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