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You are here:Home » Antibodies » Abs to Carbohydrates, Glycoproteins » Anti -F4/80, Pan Macrophages (Cell Surface Glycoprotein EMR1, Cell Surface Glycoprotein F4/80, DD7A5-7, EGF-like Module-containing Mucin-like Hormone Receptor-like 1 Precursor, EGF-TM7, EMR1 Hormone Receptor, F4/80, Gpf480, Ly71, TM7LN3) (PE)

Anti -F4/80, Pan Macrophages (Cell Surface Glycoprotein EMR1, Cell Surface Glycoprotein
F4/80, DD7A5-7, EGF-like Module-containing Mucin-like Hormone Receptor-like 1 Precursor,
EGF-TM7, EMR1 Hormone Receptor, F4/80, Gpf480, Ly71, TM7LN3) (PE)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified B IH FC
Murine F4/80 glycoprotein contains seven-transmembrane (TM7) regions, which anchor the protein in the cell membrane, and thereby shows similarity in this region to G-protein-coupled receptors. The F4/80 molecule shares overall structural homology to other members of the epidermal growth factor (EGF)-TM7 family. The antigen is detected on tissue fixed macrophages in all organs tested so far (spleen, lymph nodes, thymus, liver, skin). It is also present on Langerhans cells in the skin and Kupffer cells in the liver. It is absent on granulocytes, lymphocytes and thrombocytes. The expression of F4/80 increases upon maturation of macrophage precursors in bone marrow and blood as well as in ontogeny. This monoclonal antibody is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation processes in the pancreas. Furthermore it is a unique histological marker of the progression from peri-insulitis to beta-cell destruction and diabetes in a mouse diabetes model.
Catalog #F0001-51D
ApplicationsSuitable for use in Flow Cytometry, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended DilutionFlow Cytometry: 10ul/250,000 cells in a 100ul total staining volume
Immunohistochemistry (Frozen): tissue embedded in OCT Tissue Tec; fixed with acetone for 10min at RT; incubation with 0.02M sodium azide in PBS containing 0.1% H2O2 for 10 min at RT to destroy endogenous peroxidase; spleen as positive control
Immunohistochemistry (Paraffin): fixation in 10% neutral buffered formalin for 24h; blocking with non-immunized goat serum; microwaved for 6min in citrate buffer; splenic macrophages as positive control
Western Blot: Mouse bone-marrow derived macrophages; non-reduced; ~125kD; reduction with 2-mercaptoethanol destroys BM8 antigen
Optimal dilutions to be determined by the researcher.
Positive Control Mouse macrophages
Storage and StabilityMay be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No13A47
HostRat
SourceMouse
ConcentrationAs reported
FormSupplied as a liquid in PBS, 1% BSA, 0.02% sodium azide. Labeled with R-Phycoerythrin (PE).
PurityPurified by Protein G affinity chromatography.
ImmunogenBALB/c macrophages obtained from 14-day-old bone marrow cell cultures
SpecificityRecognizes mouse F4/80, a 125kD extracellular macrophage membrane molecule, highly restricted to mature macrophage subpopulations residing in tissue. Does not react with mouse granulocytes, mast cells, platelets, lymphocytes fibroblasts and endothelial cells. Species Crossreactivity: human (heart macrophages).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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