| FACT complex is a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. |
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| Catalog # | F0004 |
| Applications | Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. |
| Recommended Dilutions | Western Blot: 1:1000-1:2000 dilution detects FACT/cdc68 in HeLa nuclear extracts. HeLa nuclear extract lysate was resolved by electrophoresis, transferred to PVDF and probed with F0004. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP. |
| Immunoprecipitation: Reported to immunoprecipitate FACT from HeLa nuclear extracts. |
| Optimal dilutions to be determined by the researcher. |
| Included Positive Antigen Control | H1838: HeLa Nuclear Extract. Add an equal volume of 2X Laemmli reducing sample buffer to 5-10ul of extract and boil for 5 min to reduce the preparation. Load 2-5ug of reduced lysate per lane for minigels. |
| Recommended Secondary Antibodies | I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb |
| I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb |
| I1904-46Q: IgG, H&L (HRP) Pab Gt xRb |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Mouse |
| Concentration | Not Determined |
| Form | Supplied as a liquid in 0.2M Tris, pH 8.0, 0.15M sodium chloride, 0.1mM EDTA, 5mg/ml BSA, 0.05% sodium azide, before the addition of glycerol to 30%. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | GST fusion protein corresponding to residues 802–982 of mouse FACT/cdc68. |
| Specificity | Recognizes mouse FACT/cdc68, Mr 140kD. A non-speci c band is detected at ~120kD in longer exposures or when more that 5ug of nuclear extract is loaded per lane. Species Crossreactivity: human |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
