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You are here:Home » Antibodies » Abs to Replication Factors » Anti -FACT (cdc68, Facilitates Chromatin Transcription)

Anti -FACT (cdc68, Facilitates Chromatin Transcription)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
FACT complex is a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II.
Catalog #F0004
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionsWestern Blot: 1:1000-1:2000 dilution detects FACT/cdc68 in HeLa nuclear extracts. HeLa nuclear extract lysate was resolved by electrophoresis, transferred to PVDF and probed with F0004. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP.
Immunoprecipitation: Reported to immunoprecipitate FACT from HeLa nuclear extracts.
Optimal dilutions to be determined by the researcher.
Included Positive Antigen ControlH1838: HeLa Nuclear Extract. Add an equal volume of 2X Laemmli reducing sample buffer to 5-10ul of extract and boil for 5 min to reduce the preparation. Load 2-5ug of reduced lysate per lane for minigels.
Recommended Secondary AntibodiesI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
ConcentrationNot Determined
FormSupplied as a liquid in 0.2M Tris, pH 8.0, 0.15M sodium chloride, 0.1mM EDTA, 5mg/ml BSA, 0.05% sodium azide, before the addition of glycerol to 30%.
PurityPurified by immunoaffinity chromatography.
ImmunogenGST fusion protein corresponding to residues 802982 of mouse FACT/cdc68.
SpecificityRecognizes mouse FACT/cdc68, Mr 140kD. A non-speci c band is detected at ~120kD in longer exposures or when more that 5ug of nuclear extract is loaded per lane. Species Crossreactivity: human
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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