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You are here:Home » Antibodies » Abs to Coagulation Factors » Anti -Factor Va, light chain, fragment E, 74kD (HRP)

Anti -Factor Va, light chain, fragment E, 74kD (HRP)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B RIA
Factor V is a large, single chain, plasma glycoprotein which is an essential component in the blood coagulation cascade (1). During coagulation, the procofactor, factor V, is converted to the active cofactor, factor Va, via limited proteolysis by the serine protease a-thrombin (illustrated above), and less efficiently by factor Xa. The active cofactor is composed of an NH2-terminal derived heavy chain (Mr=94,000) and a COOH-terminal derived light chain (Mr=74,000) which remain non-covalently associated in the presence of calcium ions. Factor Va serves as a cofactor for the serine protease factor Xa. Factor Va and Xa assemble on a phospholipid surface in a non-covalent and calcium ion-dependent manner, to form the prothrombinase complex. The prothrombinase complex is responsible for the rapid conversion of the zymogen prothrombin to the active serine protease, a-thrombin. Assembly of the prothrombinase complex increases the rate at which prothrombin is converted to thrombin by nearly 300,000-fold relative to the rate with factor Xa alone. Down regulation of the prothrombinase complex is accomplished partly through the inactivation of factor Va by activated protein C.
Catalog #F0011-24A
ApplicationsSuitable for use in ELISA, RIA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 5ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3H368
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2. Labeled with Horseradish Peroxidase (HRP) .
PurityPurified.
ImmunogenPurified human Factor V
SpecificityRecognizes human Factor Va, light chain, (fragment E, 74,000). Does not react with intact Factor V.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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