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You are here:Home » Antibodies » Antibodies-Coagulation Factors » Anti -Factor VIII

Anti -Factor VIII


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Clone Host Grade Applications
Monoclonal Rat Purified E B
Factor VIII (formerly referred to as antihemophilic globulin and Factor VIIIc) is a large glycoprotein (320kD) that circulates in plasma at approximately 200ng/ml. Synthesized in the liver, the majority of Factor VIII is cleaved during expression resulting in a heterogeneous mixture of partially cleaved forms of F.VIII ranging in size from 200-280kD. The F.VIII is stabilized by association with von Willebrand Factor to form a F.VIII-vWF complex required for normal survival of F.VIII in vivo (T1/2 of 8-12 hours. F.VIII is a pro-cofactor that is activated through limited proteolysis by thrombin. In this process F.VIIIa dissociates from vWF to combine with activated Factor IX, calcium and a phospholipid surface where it is an essential cofactor in the assembly of the Factor X activator complex. Once dissociated from vWF, F.VIIIa is susceptible to inactivation by Protein C and by non-enzymatic decay. Hemophilia A is a congenital bleeding disorder resulting from an X-chromosome linked deficiency of F.VIII. The severity of the deficiency generally correlates with the severity of the disease. Some hemophiliacs (~10%) produce a F.VIII protein that is partially or totally inactive. The production of neutralizing antibodies to F.VIII also occurs in 5-20% of hemophiliacs.
Catalog #F0016-02
ApplicationsSuitable for use in ELISA, Western Blot and Non-inhibitory in an aPTT clotting assay. Other applications not tested.
Recommended DilutionWestern Blot: Non-reduced
Optimal dilutions to be determined by the researcher.
Extinction CoefficientE1%, 1cm, 280nm =1.4
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No7F2
FormSupplied as a liquid in dH2O, 50% glycerol.
PurityPurified. > 95% by SDS-PAGE.
ImmunogenRecombinant mouse Factor VIII
SpecificityRecognizes mouse Factor VIII.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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