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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B RIA

Human Factor VIII is an essential blood coagulation factor. Whilst circulating in the blood, it is mostly stably complexed to von Willebrand factor. It is activated through cleavage at various sites, dissociates from the complex and interacts with Factor IXa, in the presence of calcium ions and phospholipids, to convert Factor X to the activated Factor Xa, which activates thrombin. Thrombin cleaves fibrinogen into fibrin, which polymerises and cross-links to form a blood clot. The activated Factor VIII is proteolytically inactivated and cleared from the bloodstream. Defects in Factor VIII cause haemophilia A, a disorder characterised by the body’s inability to control blood clotting. This could result in severe blood loss, even with minor injuries. Clone 7E127 is a very potent coagulation inhibitor.
Catalog #	F0016-07
Applications	Suitable for use in ELISA, Western Blot and RIA. Other applications not tested.
Recommended Dilution	ELISA: 1:500-1:15,000
	Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies	I1904-04D: IgG, H&L (AP) Pab Gt xMo
	I1904-06F: IgG, H&L (FITC) Pab Gt xMo
	I1904-06C: IgG, H&L (HRP) Pab Gt xMo
	I1903-07E: IgG, (HRP) Pab Rb xMo
	I1903-13A: IgG, (PE) Pab Gt xMo
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	7E127
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, 0.09% sodium azide.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Purified human Factor VIII
Specificity	Recognizes human factor VIII. Recognizes an epitope toward the N-terminus of full-length Factor VIII and the 210kD, 90kD and 40kD cleavage products. Does not react with Von Willebrand Factor. Species Crossreactivity: porcine. Does not react with mouse, canine or rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.