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You are here:Home » Antibodies » Abs to Coagulation Factors » Anti -Factor VIII

Anti -Factor VIII

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B RIA
Human Factor VIII is an essential blood coagulation factor. Whilst circulating in the blood, it is mostly stably complexed to von Willebrand factor. It is activated through cleavage at various sites, dissociates from the complex and interacts with Factor IXa, in the presence of calcium ions and phospholipids, to convert Factor X to the activated Factor Xa, which activates thrombin. Thrombin cleaves fibrinogen into fibrin, which polymerises and cross-links to form a blood clot. The activated Factor VIII is proteolytically inactivated and cleared from the bloodstream. Defects in Factor VIII cause haemophilia A, a disorder characterised by the body’s inability to control blood clotting. This could result in severe blood loss, even with minor injuries. Clone 7E127 is a very potent coagulation inhibitor.
Catalog #F0016-07
ApplicationsSuitable for use in ELISA, Western Blot and RIA. Other applications not tested.
Recommended DilutionELISA: 1:500-1:15,000
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-04D: IgG, H&L (AP) Pab Gt xMo
I1904-06F: IgG, H&L (FITC) Pab Gt xMo
I1904-06C: IgG, H&L (HRP) Pab Gt xMo
I1903-07E: IgG, (HRP) Pab Rb xMo
I1903-13A: IgG, (PE) Pab Gt xMo
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No7E127
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified human Factor VIII
SpecificityRecognizes human factor VIII. Recognizes an epitope toward the N-terminus of full-length Factor VIII and the 210kD, 90kD and 40kD cleavage products. Does not react with Von Willebrand Factor. Species Crossreactivity: porcine. Does not react with mouse, canine or rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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