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You are here:Home » Antibodies » Antibodies-Coagulation Factors » Anti -Factor VIII, A2 Domain

Anti -Factor VIII, A2 Domain


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Clone Host Grade Applications
Monoclonal Mouse Purified E B
Hemophilia A is an X-linked, recessive, bleeding disorder caused by a deficienty in the activity of coagulation Factor VIII. Affected individuals develop a variable phenotype of hemorrhage into joints and muscles, easy bruising, and prolonged bleeding from wounds. The disorder is caused by heterogeneous mutations in the Factor VIII gene. Up to 30% of hemophilia A patients given therapeutic Factor VIII can make inhibitory antibodies, the majority of which are reactive with its C2 and A2 domains
Catalog #F0016-10E
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
HybridomaNS1 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No9L238
ConcentrationAs reported
FormSupplied as lyophilized from 20mM sodium phosphate, 0.15M sodium chloride, pH 7.4, 0.01% sodium azide. Reconstitute with 500ul ddH2O.
PurityGel filtration and anion exchange chromatograph. 98% pure (SDS-PAGE).
ImmunogenHuman Factor VIII A2 domain.
SpecificityRecognizes human factor VIII and human factor VIIIa in solid-phase ELISA and human factor VIII A2 domain in immunoblotting techniques.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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