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You are here:Home » Antibodies » Antibodies-Coagulation Factors » Anti -Factor VIII

Anti -Factor VIII


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Clone Host Grade Applications
Monoclonal Mouse Ascites E B N
Factor VIII is also known as Coagulant component, Antihemophilic factor, AHF. Factor VIII makes up only about 1% of the ciruculating FactorVIII/vWF complex with the bulk of the complex being vWF (99% by mass).
Catalog #F0016-18A
ApplicationsSuitable for use in ELISA, Western Blot. Other applications not tested.
Recommended DilutionELISA (Indirect): 1:16,000.
Western Blot: Precipitate the FactorVIII/vWF out of plasma prior to detection of Factor VIII with F0016-18A. When perfoming Western Blot of plasma preparations, background, non-specific bands, caused by the serum proteins are common. More complex blocking solutions or higher salt concentrations can help remove non-specific bands. Consult reference 2 (Rotblat, et al., Biochemistry 24(16): 4294-4300 (1985)) for additional tips with regard to detecting Factor VIII via Western Blot
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No2Q2133
ConcentrationNot determined
FormSupplied as a liquid. No preservatives added.
PurityAscites fluid
ImmunogenHuman Factor VIII
SpecificityRecognizes the light chain of Factor VIII (83kD). Does not crossreact with von Willebrand Factor. Species crossreactivity: Human. Others not tested.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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