Suitable for use in ELISA and Western Blot. Other applications not tested.

ELISA (Indirect): 1:16,000. Western Blot: Precipitate the FactorVIII/vWF out of plasma prior to detection of Factor VIII with F0016-18A. When perfoming Western Blot of plasma preparations, background, non-specific bands, caused by the serum proteins are common. More complex blocking solutions or higher salt concentrations can help remove non-specific bands. Consult reference 2 (Rotblat, et al., Biochemistry 24(16): 4294-4300 (1985)) for additional tips with regard to detecting Factor VIII via Western Blot Optimal dilutions to be determined by the researcher.

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Human Factor VIII

Supplied as a liquid. No preservatives added.

Ascites

Recognizes the light chain of human Factor VIII (83kD). Does not crossreact with von Willebrand Factor.