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Specifications

Clone Host Grade Applications Monoclonal Mouse Ascites E B N

Factor VIII is also known as Coagulant component, Antihemophilic factor, AHF. Factor VIII makes up only about 1% of the ciruculating FactorVIII/vWF complex with the bulk of the complex being vWF (99% by mass).
Catalog #	F0016-18A
Applications	Suitable for use in ELISA, Western Blot. Other applications not tested.
Recommended Dilution	ELISA (Indirect): 1:16,000.
	Western Blot: Precipitate the FactorVIII/vWF out of plasma prior to detection of Factor VIII with F0016-18A. When perfoming Western Blot of plasma preparations, background, non-specific bands, caused by the serum proteins are common. More complex blocking solutions or higher salt concentrations can help remove non-specific bands. Consult reference 2 (Rotblat, et al., Biochemistry 24(16): 4294-4300 (1985)) for additional tips with regard to detecting Factor VIII via Western Blot
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	2Q2133
Host	Mouse
Source	Human
Concentration	Not determined
Form	Supplied as a liquid. No preservatives added.
Purity	Ascites fluid
Immunogen	Human Factor VIII
Specificity	Recognizes the light chain of Factor VIII (83kD). Does not crossreact with von Willebrand Factor. Species crossreactivity: Human. Others not tested.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.