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You are here:Home » Antibodies » Abs to Coagulation Factors » Anti -Factor VIIIc (Antihaemophilic Factor)

Anti -Factor VIIIc (Antihaemophilic Factor)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Purified E N
Factor VIII (antihemophilic globulin and Factor VIII:C) is a large glycoprotein (320kD) that circulates in plasma at ~200ng/ml. Synthesized in the liver, the majority of Factor VIII is cleaved during expression, resulting in a heterogeneous mixture of partially cleaved forms of F.VIII ranging in size from 200-280kD. The F.VIII is stabilized by association with von Willebrand Factor to form a F.VIII-vWF complex required for the normal survival of F.VIII in vivo (t1/2 of 8-12 hours). F.VIII is a pro-cofactor that is activated through limited proteolysis by thrombin. In this process F.VIIIa dissociates from vWF to combine with activated Factor IX, calcium and a phospholipid surface where it is an essential cofactor in the assembly of the Factor X activator complex. Once dissociated from vWF, F.VIIIa is susceptible to inactivation by activated Protein C and by non-enzymatic decay.
Hemophilia A is a congenital bleeding disorder resulting from an X-chromosome-linked deficiency of F.VIII. The severity of the deficiency generally correlates with the severity of the disease. Some Hemophiliacs (~10%) produce a F.VIII protein that is partially or totally inactive. The production of neutralizing antibodies to F.VIII also occurs in 5-20% of Hemophiliacs (1-3).
Catalog #F0016-30
ApplicationSuitable for use in ELISA, Immunoelectrophoresis and Neutralization. Other applications not tested.
Recommended DilutionNeutralization: 420 Bethesda Units/ml IgG against normal plasma (4). One Bethesda unit/ml is defined as the amount of inhibitor that resulted in 50% residual F.VIII activity after 2 hours at 37ºC.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
Concentration~10mg/ml (OD280nm, E1%=13.4)
FormSupplied as a liquid in 10mM HEPES, pH 7.4, 0.15M sodium chloride, 50% glycerol.
PurityPurified by caprylic acid and ammonium sulfate precipitations, and solid-phase adsorption to remove unwanted crossreactivity.
ImmunogenHuman Factor VIIIC (F.VIII) purified from concentrate
SpecificityRecognizes human F.VIII as demonstrated by immunodiffusion and ELISA. No reactivity was observed with von Willebrand Factor.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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